Coding

Part:BBa_K843000:Design

Designed by: Berke Gürkan   Group: iGEM12_METU   (2012-09-26)


Carbon Monoxide Dehydrogenase - Mycobacterium bovis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1288
    Illegal BamHI site found at 351
    Illegal BamHI site found at 1247
    Illegal BamHI site found at 1805
    Illegal BamHI site found at 2591
    Illegal XhoI site found at 3132
    Illegal XhoI site found at 3552
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 73
    Illegal NgoMIV site found at 731
    Illegal NgoMIV site found at 858
    Illegal NgoMIV site found at 2396
    Illegal NgoMIV site found at 3240
    Illegal NgoMIV site found at 3653
    Illegal AgeI site found at 1750
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1902
    Illegal BsaI.rc site found at 2685
    Illegal BsaI.rc site found at 3570


Design Notes

The sequence of the part is changed and six bases are added in order to get rid of an XbaI cut site.


Source

Medium chain : http://www.ncbi.nlm.nih.gov/gene/11808648

Small chain: http://www.ncbi.nlm.nih.gov/gene/11808647

Large chain: http://www.ncbi.nlm.nih.gov/gene/11808646

References